Figure 5.

Point mutation at Cys¹⁸³ partially prevents S-nitrosylation of ERK1 in glioma cells. (A) Location of Cys and the adjacent amino acids in the ERK1 protein sequence. The red 'C' in the middle of each box is cysteine residue. (B) Transfection efficiency and S-nitrosylation of ERK1^WT and ERK1^C183A were determined by biotin switch assay, followed by western blotting. (C) Semi-quantitative analysis of ERK1-SNO levels. β-actin was used as a loading control. ^*P<0.05 compared with the ERK1^WT group. Cys, cystein; ERK, extracellular signal-regulated kinase; SNO, S-nitrosothiol; IB, immunoblotting; WT, wild-type.