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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Am J Transplant. 2017 Nov 23;18(3):604–616. doi: 10.1111/ajt.14543

Table 1. Sequences of primer sets used for Real-time Quantitative PCR.

Forward primer Reverse primer
NOS2 5′-gtt ctc agc cca aca ata caa ga-3′ 5′-gtg gac ggg tcg atg tca c-3′
Tnfa 5′-gcc tct tct cat tcc tgc ttg-3′ 5′-ggg tct ggg cca tag aac tg-3′
Il1b 5′-aag ggc tgc ttc caa acc ttt gac-3′ 5′-ata ctg cct gcc tga agc tct tgt-3′
ARG1 5′-ctc caa gcc aaa gtc ctt aga g-3′ 5′-agg agc tgt cat tag gga cat c-3′
CD206 5′-ttg gac gga tag atg gag gg-3′ 5′-cca ggc agt tga gga ggt tc-3′
Chi3l3 5′-tca ctt aca cac atg agc aag ac-3′ 5′- cgg ttc tga gga gta gag acc a-3′
DC-sign 5′-gaa gag cag aac ttt cta-3′ 5′-tca tga aac tga gag tca gag-3′
PD-L1 5′-caa tga gaa tgc tag atg tg-3′ 5′-tcc atc ttg agt ctt tgg ac-3′
PD-L2 5′-gta ccg ttg cct ggt cat ct-3′ 5′- gcc agg aca ctt ctg cta gg-3′
Fgr2b 5′-caa aac tga ggc tga gaa tac-3′ 5′-aat atc tac agc atc cct tgg-3′
Nrf2 5′-cat tcc cga att aca gtg tc-3′ 5′-gga gat cga tga gta aaa atg g-3′
Sirpa 5′-aaa taa ccc aga tcc agg ac-3′ 5′-ttg cat att ctg tgt ggt tg-3′
CD300Lg 5′-gaa cct cag tca gtc tac ag-3′ 5′-tct gca att aca cag aga tg-3′
Clec12a 5′-aca gct gtt att ctc aac tc-3′ 5′-taa att cca gca cat cct tg-3′
Hif1a 5′-cga tga cac aga aac tga ag-3′ 5′-gaa ggt aaa gga gac att gc-3′
Lair1 5′-ctg agc ctt ata aaa cag agg-3′ 5′-caa gat gta tct gag gtt gg-3′
Pirb 5′-tca gga aag atg tcc aga aag aga-3′ 5′-gct gtt cag ctc cac tcc at-3′
Trem2 5′-tca tct ctt ttc tgc act tc-3′ 5′-tca taa gta cat gac acc ctc-3′
Trem3 5′-cta cct ctc tcc tga caa tg-3′ 5′-cag aga aag aca aac agg ac-3′
Vtcn1 5′-cct tga gta taa gac cgg ag-3′ 5′-tgg tca cat tct cag agt tc-3′
ICOSLg 5′-gac aat agc cta ata gac acg-3′ 5′-ttt cca gtg aaa ctt tct-3′
Siglec-1 5′-cta gac ttc tat gct aat gtgg-3′ 5′-gga tcc ttc cag aag tag ag-3′
HPRT 5′-agt aca gcc cca aaa tgg tta-3′ 5′-ctt agg ctt tgt att tgg ctt t-3′