Effect of AM4113 on ethanol consumption and preference was examined using a two-bottle choice paradigm. AM4113 significantly suppressed ethanol consumption at dose 1 mg/kg on day 1 (***p<0.0001) and 2 (*p<0.05), and at dose 3 mg/kg on day 1 (***p<0.0001), day 2(***p<0.0001), day 3 (*p<0.05) and day 4 (*p<0.05). A marked reduction in ethanol preference was observed at dose 1 mg/kg on day 1 (***p<0.0001) and 2 (*p<0.05), and at dose 3 mg/kg on day 1 (***p<0.0001), day 2 (***p<0.0001), day 3 (*p<0.05) and day 4 (*p<0.05). Data are presented as mean ± SEM. (p values are relative to the vehicle treated group (Vehicle = 10; 1 mg/kg =10; 3 mg/kg = 11); Two-way mixed ANOVA with Bonferroni post-hoc test).