The concentrations of AM4113 in brain and plasma were analyzed by liquid chromatography-mass spectrometry (LC-MS) following intraperitonial injection of mice at dose of 1 mg/kg. Plasma (A; ***p<0.0001 at 20 min [n=4] compared to 40 min [n=5], 60 min [n=5], 90 min [n=5], 180 min [n=5], 300 min [n=4] and 420 min [n=3] post-injection time points) and brain (B; *p<0.05; **p<0.001;***p<0.0001 compared to 10 min post-injection time point) concentrations of AM4113 were significantly higher at 20 min and 60 min, respectively.