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. Author manuscript; available in PMC: 2019 Feb 20.
Published in final edited form as: Biochemistry. 2018 Jan 31;57(7):1178–1189. doi: 10.1021/acs.biochem.7b01132

Figure 2.

Figure 2

A new class of genetically encoded probes. Panel A: a flavoenzyme (yellow) with strong fluorescence in its oxidized state is fused to a GFP variant (red cylinder). Interaction with a specific substrate of the flavoenzyme ablates the flavin fluorescence and provides for analyte sensing. Two modes of analysis are depicted in panels B and C. In the ratiometric mode the GFP analog is used to normalize for net concentration and spatial presentation of the probe within the sample. Suitable matching of fluorophores permits a significant FRET signal as depicted in panel C.