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. Author manuscript; available in PMC: 2019 Feb 20.
Published in final edited form as: Biochemistry. 2018 Jan 31;57(7):1178–1189. doi: 10.1021/acs.biochem.7b01132

Figure 6.

Figure 6

Reduction of TrxRGV-mCherry in vitro and in E. coli. Panel A shows the fluorescence of 1 μM probe mixed at 50 s with 10 mM of GSH or DTT or with 0.1 mM NADPH. Reduction for DTT and NADPH show half-times of 37 ± 2 s and 48 ± 2 s respectively analyzed by exponential fit. Panel B represents the fluorescence of E. coli cells expressing the thioredoxin probe before and after the addition of the indicated reagents. The lower lines represent a corresponding E. coli strain lacking the plasmid for TrxRGV-mCherry.