Figure 2. The endogenous TLR4 ligand Fn^EDA induces TLR4-dependent myofibroblasts differentiation via TLR4.

Skin fibroblasts were incubated with fn^EDA for 72 h in absence or presence of the novel MD2-TLR4 inhibitor T5342126. Immunofluorescence using antibodies to ASMA demonstrating increased myofibroblasts differentiation and stress fiber formation which was completely abrogated by T5342126 treatment. Representative images. Scale bar, 10 μm.