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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Transplantation. 2018 Mar;102(3):396–405. doi: 10.1097/TP.0000000000002010

Figure 2. Immunophenotyping of B cell deficient rats demonstrated a lack of B cell populations and a lack of antibody production.

Figure 2

A and B, Flow cytometry demonstrated absence of B cells in lymphoid tissues in B−/− rats. Heterozygous (B+/−) and wild type (WT) rats demonstrated similar B cell numbers. Systemic levels of IgM (C) and IgG (D) were undetectable in B−/− rats as measured by ELISA. WT and heterozygous rats had similar levels of circulating IgM and IgG. Similarly, splenocytes did not produce IgM or IgG as determined by ELISPOT analysis (E and F, respectively). * P < 0.05, ** P < 0.001, *** P < 0.0001 for WT compared to B−/−.