Table 1.
Gene symbol | Gene name | Reference Mouse Homolog | Primer (5′−3′) | Product size | Correlation coefficients (R2) | PCR Efficiency (%) |
---|---|---|---|---|---|---|
ACTB | Beta-actin | 45 | F: TTGCTGACAGGATGCAGAAG | 145 | 0.9996 | 98.8 |
R: ATCCACATCTGCTGGAAGGT | ||||||
GAPDH | Glyceraldehyde 3-phosphate dehydrogenase | 46 | F: AACTTTGGCATTGTGGAAGGA | 130 | 0.9911 | 105 |
R: AACATCATCCCTGCTTCCAC | ||||||
Bet1 | Bet1 golgi vesicular membrane trafficking protein | ENSMUSG00000032757 | F: GGACACCACAGCTATGCCAA | 124 | 0.9981 | 92.7 |
R: CTTCGTGCCCGATTTCGATG | ||||||
Pdap1 | PDGFA associated protein 1 | ENSMUSG00000029623 | F: CCTCCGTTGCATCCAGACTC | 114 | 0.9736 | 106.33 |
R: TGGCTGCACAGAAGTACCAG | ||||||
Hmg20a | High mobility group 20 A | ENSMUSG00000032329 | F: CGCTACCTGGAAGTGGAGAG | 126 | 0.9903 | 108.24 |
R: GCACCAGTCACTAGCGATCA | ||||||
Ndufaf3 | NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 3 | ENSMUSG00000070283 | F:CTACCTGGAAGTGGAGAGACC | 149 | 0.9953 | 108.15 |
R: AGTCACTAGCGATCAAGCCT | ||||||
Chchd2 | Coiled-coil-helix-coiled-coil-helix domain containing 2 | ENSMUSG00000070493 | F:AGACCAGTCTGAAAGGAAACCAT | 111 | 0.9914 | 102.39 |
R:GGTGCTGAGCTGTCAGATTGTA | ||||||
Grk2 | G protein-coupled receptor kinase 2 | ENSMUSG00000024858 | F: TTACGCCCTTGGCAAAGACT | 95 | 0.9991 | 107.9 |
R: ACAGGTAGAAGTAACGCCGC | ||||||
Nckap1l | NCK associated protein 1 like | ENSMUSG00000022488 | F: AACCATGATCAACCCTGCCA | 111 | 0.9961 | 101.61 |
R: TTAAGGCACCCATGGCACAA | ||||||
Stx12 | Syntaxin 12 | ENSMUSG00000028879 | F: CAAGTCCTAGTCACCAGCGG | 106 | 0.9975 | 97.24 |
R: AAGAGTGTGTCGGCATCTCC | ||||||
Tmem97 | Transmembrane protein 97 | ENSMUSG00000037278 | F: CAGCAGCTGCAGGCTCTAAT | 118 | 0.9963 | 104.44 |
R: TGCAGAGCCTAACAGCCAAA | ||||||
Sec. 22b | SEC. 22 homolog B, vesicle trafficking protein | ENSMUSG00000027879 | F: TAGCATTGACTAGGGCATTC | 145 | 0.9941 | 94.94 |
R: CATCAGGAATGTTCATTGGC | ||||||
Smap2 | Small ArfGAP 2 | ENSMUSG00000032870 | F: TAGGACCTGACAACTGATCT | 110 | 0.9887 | 90.94 |
R: CATGACACGTGAAACTTCTG |