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. 2018 Feb 20;8:3364. doi: 10.1038/s41598-018-21723-0

Table 1.

Description of primers used in this study for RT-qPCR analysis.

Gene symbol Gene name Reference Mouse Homolog Primer (5′−3′) Product size Correlation coefficients (R2) PCR Efficiency (%)
ACTB Beta-actin 45 F: TTGCTGACAGGATGCAGAAG 145 0.9996 98.8
R: ATCCACATCTGCTGGAAGGT
GAPDH Glyceraldehyde 3-phosphate dehydrogenase 46 F: AACTTTGGCATTGTGGAAGGA 130 0.9911 105
R: AACATCATCCCTGCTTCCAC
Bet1 Bet1 golgi vesicular membrane trafficking protein ENSMUSG00000032757 F: GGACACCACAGCTATGCCAA 124 0.9981 92.7
R: CTTCGTGCCCGATTTCGATG
Pdap1 PDGFA associated protein 1 ENSMUSG00000029623 F: CCTCCGTTGCATCCAGACTC 114 0.9736 106.33
R: TGGCTGCACAGAAGTACCAG
Hmg20a High mobility group 20 A ENSMUSG00000032329 F: CGCTACCTGGAAGTGGAGAG 126 0.9903 108.24
R: GCACCAGTCACTAGCGATCA
Ndufaf3 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 3 ENSMUSG00000070283 F:CTACCTGGAAGTGGAGAGACC 149 0.9953 108.15
R: AGTCACTAGCGATCAAGCCT
Chchd2 Coiled-coil-helix-coiled-coil-helix domain containing 2 ENSMUSG00000070493 F:AGACCAGTCTGAAAGGAAACCAT 111 0.9914 102.39
R:GGTGCTGAGCTGTCAGATTGTA
Grk2 G protein-coupled receptor kinase 2 ENSMUSG00000024858 F: TTACGCCCTTGGCAAAGACT 95 0.9991 107.9
R: ACAGGTAGAAGTAACGCCGC
Nckap1l NCK associated protein 1 like ENSMUSG00000022488 F: AACCATGATCAACCCTGCCA 111 0.9961 101.61
R: TTAAGGCACCCATGGCACAA
Stx12 Syntaxin 12 ENSMUSG00000028879 F: CAAGTCCTAGTCACCAGCGG 106 0.9975 97.24
R: AAGAGTGTGTCGGCATCTCC
Tmem97 Transmembrane protein 97 ENSMUSG00000037278 F: CAGCAGCTGCAGGCTCTAAT 118 0.9963 104.44
R: TGCAGAGCCTAACAGCCAAA
Sec. 22b SEC. 22 homolog B, vesicle trafficking protein ENSMUSG00000027879 F: TAGCATTGACTAGGGCATTC 145 0.9941 94.94
R: CATCAGGAATGTTCATTGGC
Smap2 Small ArfGAP 2 ENSMUSG00000032870 F: TAGGACCTGACAACTGATCT 110 0.9887 90.94
R: CATGACACGTGAAACTTCTG