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. 2018 Feb 20;8:3372. doi: 10.1038/s41598-018-21638-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Fabrication procedure of high pressure PDMS flow chambers. (1–2) We design a customized channel scaffold using Autodesk Inventor Professional 2017 and translate the scaffold design into G-code using a slicing software. (3–4) We print the channel scaffold using a 3D printer using a water-soluble printing filament. (5–6) After curing a thin layer of PDMS on the bottom of a petri dish, we transfer and center the printed channel scaffold on the former using a pair of tweezers. (7–8) To finish the molding procedure, we pour and cure a second layer of PDMS on top of the scaffold. To dissolve the channel scaffold, we sonicate the flow chamber in a water bath. (b) Fabrication procedure of PDMS flow channels on a coverslip. Fabrication step 1–2 is identical to the procedure above. However, in (3–4) we now print the channel scaffold directly on a coverslip. To remove shape irregularities in channel shape, (5) we heat the printout on the coverslip on a hot plate. (6–7) To embed the channel scaffold, we clamp the printed coverslip in a custom-made molding device and add PDMS on top. After curing, we remove the coverslip/scaffold/PDMS assembly from the molding device and dissolve the channel scaffold in an ultrasonic water bath.