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. 2018 Feb 20;8:3375. doi: 10.1038/s41598-018-21659-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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AC026166.2-001 suppresses cell migration and induces cell cycle arrest in the G0/G1 phase of LSCC cells. (A) Cell cycles determined in LV-5-NC and LV5-AC026166.2-001-transfected AMC-HN-8 and TU-212 cells were analyzed by flow cytometry. (B) Cell apoptosis determined in LV-5-NC and LV5-AC026166.2-001-transfected AMC-HN-8 and TU-212 cells was analyzed by flow cytometry using Annexin V/PI staining. (C) Cell cycles determined in siRNA-NC and AC026166.2-001-siRNA-1-transfected AMC-HN-8 and TU-212 cells were analyzed by flow cytometry. (D) After deletion of AC026166.2-001, cell apoptosis was measured by flow cytometry using Annexin V/PI staining. (E) Wound healing analysis and transwell migration assays (F) were used to determine cell migration in LV-5-NC and LV5-AC026166.2-001-transfected AMC-HN-8 and TU-212 cells (Magnification × 40). (G) Wound healing analysis and transwell migration assays (F) were used to determine cell migration in siRNA-NC and AC026166.2-001-siRNA-1-transfected AMC-HN-8 and TU-212 cells (Magnification × 40). Data are presented as the mean ± SD, n = 3. *P < 0.05, **P < 0.01, ***P < 0.001.