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. 2018 Feb 20;8:3375. doi: 10.1038/s41598-018-21659-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

AC026166.2-001 targets miR-24-3p and promotes p27/Kip1 expression. (A) miRDB: miR-3127-5p, miR-1324, miR-3677-3P, miR-4261, miR-4496, miR-4330, miR-24-3p, miR-219a-2-3p, miR-4727-3p, miR-494-3p. miRcode: miR-190, miR-190a, miR-190b, miR-205, miR-205a, miR-205b, miR-23a, miR-23b, miR-23c, miR-23b-3p, miR-24, miR-24a, miR-24a, miR-24b, miR-24-3p. Both: miR-24-3p. (B) Bioinformatics analysis showed 7 bases in the matched binding sites between AC026166.2-001 and miR-24-3p. As shown above, binding sites or mutated sequence were used for creating firefly luciferase reporter constructs. (C) There are 4 groups (AC026166.2-001 WT/MUT 3′-UTR reporter + miR-24-3p mimics/miR-NC) for the luciferase reporter assay and results demonstrated that miR-24-3p inhibited the AC026166.2-001 WT but not the MUT. (D) After overexpression of AC026166.2-001, miR-24-3p was down-regulated in AMC-HN-8 and TU-212 cell lines. (E) Protein levels of p27, cyclin D1 in AMC-LHN-8 and TU-212 cells transfected with lentiviruses LV5-AC026166.2-001 or LV5-NC determined by Western blot analysis. For details, see Supplementary Figure S1.