Fig. 7.

Blockade of basophil accumulation in SLO dampens lupus-like disease activity. a, b Proportion of CD19⁺CD138⁺ short-lived plasma cells among living CD45⁺ cells in lymph nodes (a) and in spleen (b) from mice as described in Fig. 6b, determined by flow cytometry as described in Supplementary Fig. 8. c, d dsDNA-specific IgG levels (optical density (O.D.) at 450 nm×10²) (c) and total IgE levels (d) in blood from mice as in (a) as measured by ELISA. e, f IL-4 (e) and IL-1β (f) concentrations of total kidney protein extracts from mice as in (a), measured by ELISA. g−i Representative immunofluorescence staining for C3 and IgG deposits in kidneys from aged Lyn^–/– mice treated or not (vehicle) with both PTGDR antagonists (scale bar = 500 µm) (g) and their quantifications in mice as in (a) (h, i). a–g Data represent two pooled independent experiments. Data are presented as mean ± s.e.m. Statistical analyses were by unpaired Student t tests. NS: not significant, ^#P = 0.06, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Comparison to control group is shown above each bar and to the corresponding bars when indicated. A.U. arbitrary units