Figure 1.

Core PCP pathway components are expressed in osteoblasts and osteoclasts. (A) Schematic illustration of components of the mammalian PCP pathway (based on Nikolopoulou et al.²¹). (B) Normalised detection levels of mammalian PCP components in bone marrow and tibial cortical bone from an RNA-Seq dataset previously reported by Ayturk et al.³². (C) qRT-PCR quantification of the core PCP components Vangl1, Vangl2, Celsr1 and Scrib in osteoblast cultures differentiated for 14 or 21 days, compared with osteoblasts cultured for 7 days (set at 1 for each set of cultures). (D) qRT-PCR quantification of Vangl1, Vangl2, Celsr1 and Scrib in mature resorbing osteoblasts versus mature osteoclasts (set at 1 for each culture). N = 4–6, *p < 0.05, **p < 0.01.