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. 2018 Feb 20;9:728. doi: 10.1038/s41467-018-03135-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Localization of LEM-3 on chromatin bridges and at the midbody. a Localization of YFP-LEM-3 in wild-type embryo. b Localization of YFP-LEM-3 on chromatin bridges generated by IR, hydroxyurea, and RNAi depletion of replicative helicase subunit MCM-7, condensin II component HCP-6 and topoisomerase TOP-2. c Representative OMX images of YFP-LEM-3 localization at different stage of chromosome segregation in the presence of chromatin bridges induced by mcm-7 RNAi. Scale bars: 2 μm. d Localization of LEM-3 on chromatin bridges induced by mcm-7 RNAi. Arrows indicate YFP-LEM-3. Arrowheads indicate the chromatin bridges. Times are relative to anaphase onset of the first division. e, f Quantification of the average time duration of GFP-LEM-3 localization at the midzone/midbody during the first mitotic cell division in wild type, mcm-7 RNAi (e) and capg-1 RNAi (f) embryos. Error bars represent standard deviation of the mean. g, h Quantification of the intensity of GFP-LEM-3 foci during the first mitotic division in wild type, mcm-7 RNAi (g) (measured at 2.5 min after first appearance of LEM-3 foci) and capg-1 RNAi (h) embryos. Asterisks indicate statistical significance as determined by two-tailed Student t-test. p values below 0.05 were consider significant, where p < 0.05 was indicated with *, p < 0.01 with **, and p < 0.001 with ***