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. 2018 Feb 20;9:728. doi: 10.1038/s41467-018-03135-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Central spindle formation is required for midbody localization of LEM-3. a LEM-3 co-localized with the central spindle component ZEN-4. Arrows indicate YFP-LEM-3. b Schematic of LEM-3 localization in control embryos (normal central spindle formation) and cyk-4, spd-1 RNAi embryos (defective central spindle formation). c Midbody localization of LEM-3 depends on the central spindle component CYK-4 and the microtubule-bundling protein SPD-1. Depletion of the central spindle by cyk-4 RNAi, no LEM-3 foci are detectable even in the presence of chromatin bridges generated by mcm-7 RNAi. Images were taken from a time-lapse recording of the cyk-4 RNAi, spd-1 RNAi, and cyk-4 combined with mcm-7 RNAi-treated embryos expressing YFP-LEM-3 and mCherry-H2B from the anaphase onset of the first mitotic division. Arrowheads indicate chromatin bridges. Times are relative to anaphase onset