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. 2018 Feb 20;9:728. doi: 10.1038/s41467-018-03135-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — LEM-3 contributes to chromatin-bridge resolution. a Chromosome segregation in wild type and lem-3 mutant embryos upon partial depletion of DNA replication helicase subunit MCM-7. Images were taken from embryos expressing mCherry-H2B fed on 10% mcm-7 RNAi producing bacteria. Arrowheads indicate chromatin bridges. Times are relative to anaphase onset of the first division. b Quantification of chromatin bridges that persist into the next cell division in wild type and lem-3 mutant embryos upon full depletion of MCM-7 (100% mcm-7 RNAi). n sample size. c Persistent capg-1 RNAi induced chromatin bridges in lem-3 mutants. Chromosome segregation in wild type and lem-3 mutant embryos upon depletion of condensin I subunit CAPG-1 (100% capg-1 RNAi). Images were taken from control and capg-1 RNAi embryos expressing mCherry-H2B and GFP-PH. d, e Progeny viability of lem-3 mutants upon partial depletion of MCM-7 (d) and CAPG-1 (e). f Genetic interaction between LEM-3 and SLX-4. Error bars represent standard deviation of the mean. g LEM-3 and BRC-1 are required for faithful chromosome segregation after IR. White arrowheads indicate chromosome bridges, red arrowheads indicate micronuclei. Stars indicate polar bodies