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Long EU pulses. Arabidopsis 4 days old seedlings were incubated overnight avoiding direct light with an increasing concentration of EU (Jena Bioscience) and EU-containing RNA was detected by the click iT reaction. The last image in the row shows the result of a modified detection protocol using AF488 picolyl azide instead of AF488 azide. Confocal sections in the middle part of the root are shown. Bar = 10 μm.
