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. 2018 Feb 16;9:177. doi: 10.3389/fpls.2018.00177

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Copyright © 2018 Dvořáčková and Fajkus.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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EU signal diminishes after Actinomycin D (ActD) treatment. (A) Arabidopsis 4 days old seedlings were incubated without pre-treatment for 2 h with 1 mM EU (Invitrogen, green) avoiding direct light, detected by click iT reaction and stained with DAPI (magenta). Confocal sections in the middle part of the root are shown. Bar = 10 μm. (B) Arabidopsis 4 days old seedlings were treated for 2 h with ActD prior to EU labeling. DAPI (magenta), EU (green). Selected sections from confocal Z-stacks are shown. Bar = 10 μm.