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. 2018 Feb 16;12:9. doi: 10.3389/fnana.2018.00009

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Copyright © 2018 Fish, Rocco and Lewis.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Subtypes of CB, CR and PV neurons are distinguishable by the expression of GAD65 mRNA in human PFC.(A,B) Single plane image of a PFC tissue section labeled for CB, GAD65 and GAD67 mRNAs. (A5) and (B5) are merged images of (A1–A3) and (B1–B3), respectively. Lipofuscin autofluorescence is shown in (A4,B4). (C,D) Single plane image of a PFC tissue section labeled for CR, GAD65 and GAD67 mRNAs. (C5) and (D5) are merged images of (C1–C3) and (D1–D3), respectively. Lipofuscin autofluorescence is shown in (C4,D4). (E,F) Single plane image of a PFC tissue section labeled for PV, GAD65 and GAD67 mRNAs.(E5) and (F5) are merged images of (E1–E3) and (F1–F3), respectively. Lipofuscin autofluorescence is shown in (E4,F4). In all images, the outline of the nucleus, which was visualized using DAPI, is shown. Scale bars = 5 μm.