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. 2018 Feb 16;12:9. doi: 10.3389/fnana.2018.00009

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Copyright © 2018 Fish, Rocco and Lewis.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The proportion of three CB/GAD+ terminal subpopulations differs across cortical layers. (A) Projection image (6 z-planes separated by 0.25 μm) of a human PFC tissue section immunolabeled for CB, vGAT (not shown), GAD65 and GAD67. Gray scale images (A1–A3) are single channel images of the multichannel image (A4) and are representative of the immunohistochemistry labeling. Scale bar = 5 μm. (B,C) Bar graphs showing the relative proportions of vGAT/CB+ terminals in PFC that were GAD65+, GAD67+, or GAD65/GAD67+. Error bars = SEM.