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. 2018 Feb 16;6:14. doi: 10.3389/fcell.2018.00014

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Copyright © 2018 Prabhudesai, Koceja, Dey, Eisa-Beygi, Leigh, Bhattacharya, Mukherjee and Ramchandran.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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cbsb morphant phenotype at 52 hpf. Low magnification images of 52 hpf Tg (flk:EGFP) embryos injected with 2 ng CBSB-S1 (A) and 2 ng control (Con) MO are shown. Panel (C) shows various phenotypes observed from mild to server for CBSB-S1 at 52 hpf. Note the progressive loss of axis length. Panel (D) shows quantification of the length of the axis is shown in (A). Y-axis shows mean length of the embryo measured as the longest horizontal dimension between the start of the yolk and the end of the tail (n = 11 for CBSB-S1 and ConMO-injected embryos), ^****P < 0.0001. In the figure panels, the embryo orientation is left is anterior (A) and right is posterior (P) while top is dorsal (D) and bottom is ventral (V). Scale bars indicate 0.25 mm.