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. 2017 Feb 7;55(2):1477–1487. doi: 10.1007/s12035-017-0422-9

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© The Author(s) 2017

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 3 — Glucose deprivation alters the accumulation of lipid droplets. Accumulation of lipid droplets was quantified by staining with BODIPY 493/503 and integration of green fluorescence within the soma of microglia identified by Hoechst 33342 and CD68 labelling, and is indicative of an increased size and/or number of lipid droplets. Fluorescence was significantly increased over the course of 48 h in the normal glucose condition, but not during glucose deprivation. Both normal and glucose-deprived cells showed significant increases in BODIPY 493/503 staining after LPS stimulation at either 24 or 48 h. Asterisk indicates significant groupwise differences by two-way ANOVA between control and LPS and Double asterisk indicates pairwise significance by Bonferroni’s post hoc (N = 4)