Fig. 4.

Cultured hippocampal neurons develop synapses in the absence of WT ELOVL4 with formation of both excitatory glutamatergic and inhibitory GABAergic synapses Hippocampal neurons cultured from Elovl4 ^wt/wt and Elovl4 ^mut/mut embryos all formed pre-synaptic terminals in vitro (arrowheads) as shown by labeling for SV2 (a, b), a ubiquitous synapse marker; VGluT1 (c, d), a marker for glutamatergic terminals; and GAD-65 (e, f), a marker for GABAergic terminals. Double labeling for VGluT1 and the pre-synaptic active zone (AZ) confirmed glutamatergic synapse formation (arrowheads) by hippocampal neurons cultured from Elovl4 ^wt/wt and Elovl4 ^mut/mut embryos (g–l). Double labeling for GAD-65 and the pre-synaptic active zone confirmed GABAergic synapse formation (arrowheads) by hippocampal neurons cultured from Elovl4 ^wt/wt and Elovl4 ^mut/mut embryos (m–r). Scale bars = 20 μm for all panels