Fig. 6.

Altered hippocampal network properties and spontaneous activity in Elovl4 ^mut/mut mice. Extracellular electrophysiology under physiological conditions in hippocampal slices ex vivo collected from S ⁺ Elovl4 ^wt/wt and S ⁺ Elovl4 ^mut/mut mice. The following measurements were made during 600 trace (1 s/trace) recordings of extracellular field potentials in hippocampal slices perfused with normal ACSF at 37 °C (see also suppl. Video 3). a Spontaneous frequency as a measure of spikes/s. b Spontaneous inter-spike interval (ISI) as a measure of time between spikes. c “Active” spontaneous frequency as a measure of spikes/s of activity. d Amplitude (+) spikes as a measure of spike magnitude (mV). e Amplitude (−) spikes as a measure of spike magnitude (mV). See methods for detailed statistics (WT: n = 7, slice # = 13; mut: n = 14, slice # = 34) error ± 95% confidence interval. f The input/output ratio in response to stepwise increased stimulation (20 μA minus 100 μA). Note logarithmic normalization on y-axis (statistics: two-way RM ANOVA, *p < 0.05 from 70 to 100 μA, error ± 95% confidence interval. WT: n = 3; mut: n = 10)