Figure 2.

The effect of CDs-LP on the E. coli biofilm was assayed by the crystal violet method. The E. coli biofilm was grown at 28°C in 1/5 LB with the treatment of different concentrations of CDs-LP for different times as indicated in the figure. The absorbance of E. coli biofilm in the absence of CDs-LP at 24, 48, and 72 h after staining by the crystal violet, was recorded at 595 nm and arbitrarily assigned as 100%, respectively (A). Also, the E. coli biofilms with the treatment of 0, 0.1, 0.8, or 3 mg/mL CDs-LP for 72 h were stained by the crystal violet and photographed (B).