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HPLC chromatogram showing free phosphate release. (a) Standard of phosphate (5 mM), (b) phosphate release by PS24 as a result of solubilization of calcium triphosphate in tris minimal medium supplemented with 5 mM calcium triphosphate. Key. The peak that elutes at 30 min (indicated with an asterisk) represents phosphate. (a) Standard of phosphate. (b) Phosphate release at 10th day of incubation.
