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. 2017 Nov 6;6:1958. [Version 1] doi: 10.12688/f1000research.12985.1

Figure 4. Crosstalk between trout RBCs and the spleen stromal cell line TSS.

Figure 4.

Control and trout RBCs stimulated with VHSV UV-inactivated, multiplicity of infection (MOI) 1, were posteriorly co-culture with TSS cell line, at 14°C, and ifn1, mx, vig1 and il15 gene expression profiles were quantified at 24 hours postexposure (hpe) for RBCs and TSS. ( A) Linear regression between ifn1 and the interferon stimulated genes (ISGs) mx, vig1 and il15 gene expression profiles in RBCs. ( B) Linear regression between RBCs and TSS ifn1 gene expression profile. ( C) Linear regression between ifn1 and the ISGs mx, vig1 and il15 gene expression profiles in TSS. Gene expression was normalized against eukaryotic 18S rRNA and relativized to control cells (red line) (fold of increase). Data is displayed as the linear regression line between the indicated cell lines and expressed genes (r 2: coefficient of determination, asterisk denote statistical significance, P-value < 0.05) (n = 6). ( D) Schematic representation of the RBCs and TSS co-culture assay and analysis.