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. 2018 Feb 9;6:1958. Originally published 2017 Nov 6. [Version 2] doi: 10.12688/f1000research.12985.2

Figure 10. Effect of VHSV on ROS intracellular production and antioxidant enzymes gene expression in rainbow trout RBCs.

Figure 10.

RBCs were exposed to VHSV with a multiplicity of infection (MOI) of 1 at 14°C, ( A) DCFDA (2′,7′-Dichlorofluorescin diacetate) fluorescence intensity of VHSV-exposed RBCs relative to control cells (non-exposed), 72 hours postexposure (hpe). ( B) Antioxidant genes ( fth: ferritin, gstp1: glutathione S-transferase P, nkef: natural killer enhancement factor-like protein, sod1: superoxide dismutase [Cu-Zn], trx: thioredoxin) gene expression quantified by RT-qPCR at 3 hpe (grey bars) and 72 hpe (black bars). Gene expression was normalized against eukaryotic 18S rRNA and relativized to control cells (time 0, red line) (fold-change). Data is displayed as mean ± SD (n = 3). Kruskal-Wallis Test with Dunn´s Multiple Comparison post-hoc test was performed among all conditions. Values denote pairwise significant differences ( P-value < 0.05) with the value-indicated condition.