Table 1. Primer and probe sequences.
| Gene | Forward primer
(5’ – 3’) |
Reverse primer
(5’ – 3’) |
Probe
(5’ – 3’) |
Reference or
accession number |
|---|---|---|---|---|
| ef1α | ACCCTCCTCTTGGTCGTTTC | TGATGACACCAACAGCAACA | GCTGTGCGTGACATGAGGCA | 97 |
| tlr3 | ACTCGGTGGTGCTGGTCTTC | GAGGAGGCAATTTGGACGAA | CAAGTTGTCCCGCTGTCTGCTCCTG | NM_001124578.1 |
| irf7 | CCCAGGGTTCAGCTCCACTA | GGTCTGGCAACCCGTCAGT | TCGAGCCAAACACCAGCCCCT | AJ829673 |
| ifn1 | ACCAGATGGGAGGAGATATCACA | GTCCTCAAACTCAGCATCATCTATGT | AATGCCCCAGTCCTTTTCCCAAATC | AM489418.1 |
| mx1-3 | TGAAGCCCAGGATGAAATGG | TGGCAGGTCGATGAGTGTGA | ACCTCATCAGCCTAGAGATTGGCTCCCC | 98 |
| pkr | GACACCGCGTACCGATGTG | GGACGAACTGCTGCCTGAAT | CACCACCTCTGAGAGCGACACCACTTC | NM_001145891.1 |
| il15 | TACTATCCACACCAGCGTCTGAAC | TTTCAGCAGCACCAGCAATG | TTCATAATATTGAGCTGCCTGAGTGCCACC | XM_021575070.1 |
| vig1 | CTACAATCAAGGTGGTGAACAATGT | GTGGAAACAAAAACCGCACTTATA | TCTCAAGCTTCGGCAACTCCAAGCA | XM_021582972.1 |
| hepcidin | TCCCGGAGCATTTCAGGTT | GCCCTTGTTGTGACAGCAGTT | AGCCACCTCTCCCTGTGCCGTTG | AF281354.1 |
| β-globin | CAACATCTTGGCCACATACAAGTC | TTGTCAGGGTCGACGAAGAGT | NM_001160555.2 | |
| fth | GGCGTATTACTTCGATCGTGATG | CCCTCCCCTCTGGTTCTGA | EU302524.1 | |
| gstp1 | CCCCTCCCTGAAGAGTTTTGT | GCAGTTTCTTGTAGGCGTCAGA | BT048561.1 | |
| nkef | CGCTGGACTTCACCTTTGTGT | ACCTCACAACCGATCTTCCTAAAC | U27125.1 | |
| sod1 | GCCGGACCCCACTTCAAC | CATTGTCAGCTCCTGCAGTCA | AF469663.1 | |
| trx | AGACTTCACAGCCTCCTGGT | ACGTCCACCTTGAGGAAAAC | XM_021614924.1 | |
| N VHSV | GACTCAACGGGACAGGAATGA | GGGCAATGCCCAAGTTGTT | TGGGTTGTTCACCCAGGCCGC | 35 |