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. Author manuscript; available in PMC: 2018 Feb 21.
Published in final edited form as: Stem Cells. 2016 May 27;34(8):2026–2039. doi: 10.1002/stem.2393

Figure 3.

Figure 3

CSC conditioned media increases arginase-1 production and survival in MDSCs. Schematic (A) depicting the coculture strategy employed to determine the effect of CSC and NSTC conditioned media on MDSCs. Flow cytometry plots (B) of overall MDSC levels (red region) with control (unconditioned) media (null), NSTC conditioned media, and CSC conditioned media. Arginase-1 expression in MDSCs derived from the marrow of tumor-bearing mice was elevated as assessed by flow cytometry with CSC conditioned media compared with NSTC conditioned and control media. These results were repeated using mice implanted with two separate patient-derived human GBM specimens, hGBM 4121 (C) and hGBM 10 (D). The ratio of live to dead cells as assessed by Annexin V in MDSCs derived from the marrow of tumor-bearing mice was elevated with CSC conditioned media and compared with NSTC conditioned and control media. Experiments were repeated with conditioned media generated from the CSCs and NSTCs isolated from hGBM 4121 (E) and hGBM 10 (F) xenografts. Values shown are means ±standard deviation, *, p <.05, **, p <.01, and ***, p <.001 by one-way ANOVA. Abbreviations: CM, conditioned media; CSCs, cancer stem cells; MDSC, Myeloid-derived suppressor cell; NSTC, Nonstem tumor cell.