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. Author manuscript; available in PMC: 2018 Feb 21.
Published in final edited form as: Stem Cells. 2016 May 27;34(8):2026–2039. doi: 10.1002/stem.2393

Figure 4.

Figure 4

MDSCs exposed to CSC conditioned media promote immune suppression. Schematic (A) depicting the coculture strategy employed to determine the effect of MDSCs exposed to CSCs and NSTC conditioned media on T cell function. The ratio of CD4+ to CD8+ cells (B) was altered by MDSCs exposed to CSC conditioned media compared with NSTC conditioned media. The activation of T cells as reported by interferon-γ (IFNγ) expression (C) was suppressed when MDSCs were exposed to CSC conditioned media compared with NSTC conditioned media. Values shown are means ±standard deviation, *, p <.05, **, p <.01, and ***, p <.001 by one-way ANOVA. Activated T cells and neutrophils were used as controls. Abbreviations: CSC, cancer stem cell; MDSCs, Myeloid-derived suppressor cells; NSTC, Nonstem tumor cell.