Fig. 3.

Expression of ZmLEC1 in developing transgenic Arabidopsis (a) and Camelina (b) seeds. a Total RNA was isolated from developing seeds 6–9 DAF and subjected to RT-PCR analyses. The PP2AA3 gene was used as a housekeeping gene to confirm the quality and quantity of RNA. b Total RNA was isolated from developing seeds 15 DAF. The CsEF1f gene was used as a housekeeping gene to confirm the quality and quantity of RNAs. WT is the wild type, AtSL are independent homozygous T3 lines expressing the pSCPL17:ZmLEC1 transgenes in Arabidopsis. CsAL and CsSL are independent homozygous T3 lines expressing the pSCPL17:ZmLEC1 and pACP5:ZmLEC1 transgenes, respectively, in Camelina