Figure 5.

Pioglitazone (PIO) reduced the protein levels of p53 and cyclin D1 after long-term treatment of J82 cells. A. NUTE and J82 cells were treated with 10 μmol/L or without PIO for the indicated times. Total protein was extracted for immunoblotting of p53, cyclin D1, Bcl-2, and Bax, with β-actin used as a loading control. B. The protein expression of p53 is shown as gray stripes. C. The protein expression of cyclin D1 is shown as gray stripes. D. The protein expression of Bax is shown as gray stripes. E. The protein expression of Bcl-2 is shown as gray stripes.