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. 2018 Jan 8;15(3):238–247. doi: 10.7150/ijms.22563

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Subcutaneous PRP injection decreased p-p38 and p-NFκB immunoreactivity in spinal cord dorsal horns. Spinal cord sections (10 µm) were harvested 4 weeks after PRP injection. Double immunofluorescence staining was performed for p-p38 (red), p-NFκB (red), and OX42 (green, microglia cell). The total number of pp38 and OX42 double-positive cells in the burn + PRP group decreased significantly compared with the burn group (p-p38/OX42, group C 40.76% ± 6.22% vs group D 21.72% ± 1.11%, P < 0.05). The number of p-NFκB-expressing microglia was significantly higher in the group C than in the group D (burn 21.96 ± 1.94%, burn + PRP 6.62 ± 1.51, p < 0.01). Scale bars in all images are 50 µm.