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. 2018 Jan 15;15(3):257–268. doi: 10.7150/ijms.21620

Figure 5.

Figure 5

Figure 5

Figure 5

Osteogenic differentiation of BMMSCs on different matrix stiffnesses. (A)RUNX2, COL1A1, ALP, Osteopontin and Osteocalcin gene expressions on different matrices after 4h, 24h, 72h and 1 week of differentiation. (B) RUNX2, COL1A1, ALP, Osteopontin and Osteocalcin gene expressions on 13-16 kPa, 48-53 kPa and 62-68 kPa at different time point of differentiation. (C) After cultured on three groups for 1 week, we stained Alizarin red S to detect calcium deposits. Scale bar =100μm. *P<0.05, **P<0.01.