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. 2018 Feb 20;86(3):e00651-17. doi: 10.1128/IAI.00651-17

FIG 3.

FIG 3

TLR2−/− mice exhibit deficient Th1 immune responses elicited by DnaJ-ΔA146Ply. Seven days after the last immunization, suspensions of splenocytes (1 × 105/well) were cultured and exposed to 5 μg/ml of DnaJ-ΔA146Ply for 72 h at 37°C. Levels of IFN-γ (A), IL-17A (B), and IL-4 (C) in culture medium were determined by ELISA. *, P < 0.05; **, P < 0.01; ***, P < 0.001.