TLR2−/− mice exhibit deficient Th1 immune responses elicited by DnaJ-ΔA146Ply. Seven days after the last immunization, suspensions of splenocytes (1 × 105/well) were cultured and exposed to 5 μg/ml of DnaJ-ΔA146Ply for 72 h at 37°C. Levels of IFN-γ (A), IL-17A (B), and IL-4 (C) in culture medium were determined by ELISA. *, P < 0.05; **, P < 0.01; ***, P < 0.001.