FIG 4.

DnaJ-ΔA146Ply induces phenotypic and functional maturation of DCs. (A) Immature BMDCs (1 × 10⁶/well) were treated with 5 μg/ml of DnaJ-ΔA146Ply or 100 ng/ml of LPS for 24 h and then stained with FITC-conjugated anti-CD11c and phycoerythrin (PE)-conjugated anti-CD40, -CD86, and MHC-II. The percentage of positive cells was analyzed by flow cytometry. Immature BMDCs (1 × 10⁵/well) were stimulated with LPS or 1, 5, or 10 μg/ml of DnaJ-ΔA146Ply for 24 h. Levels of TNF-α (B), IL-6 (C), IL-1β (D), IL-23 (E), and IL-12p70 (F) in culture medium were assayed by ELISA. *, P < 0.05; **, P < 0.01; ***, P < 0.001 (for LPS or DnaJ-ΔA146Ply groups compared with medium control).