FIG 6.

MAPK, NF-κB, and PI3K-Akt pathways are involved in DC maturation mediated by DnaJ-ΔA146Ply. (A) BMDCs from WT, TLR2^−/−, and TLR4^−/− mice were treated with 5 μg/ml of DnaJ-ΔA146Ply for the indicated times and lysed in lysis buffer. The phosphorylation of corresponding molecules was analyzed by Western blotting. (B and C) BMDCs from WT mice were pretreated with specific inhibitors of p38 (SB203580), ERK1/2 (U0126), Jun N-terminal protein kinase (JNK) (SP600125), PI3K (LY294002), and NF-κB (BAY11-7082) for 1 h prior to stimulation with DnaJ-ΔA146Ply. Cytokines TNF-α and IL-6 in supernatants were quantified with ELISA kits. *, P < 0.05; **, P < 0.01; ***, P < 0.001.