FIG 7.

Absence of TLR2 in DCs impairs Th1 polarization in a DC and CD4⁺ T cell coculture system. Immature BMDCs from WT and TLR2^−/− mice were treated with 5 μg/ml of DnaJ-ΔA146Ply or 100 ng/ml of LPS for 24 h and then cocultured with purified naive CD4⁺ T cells sorted from spleens of WT and TLR2^−/− mice, respectively. (A to C) After 72 h, IFN-γ, IL-17A, and IL-4 levels in culture medium were assayed by ELISA. (D to F) Intracellular cytokines IFN-γ, IL-17A, and IL-4 were analyzed by flow cytometry. *, P < 0.05 for IFN-γ level in WT CD4⁺ T cells cocultured with WT BMDCs compared to TLR2^−/− BMDCs.