Silver-stained SDS-PAGE profile of purified LPS from A. pleuropneumoniae serotype 1. Lane 1, protein markers; lanes 2 to 4, A. pleuropneumoniae LPS; lane 5, blank control with PBS. This gel showed that purified A. pleuropneumoniae LPS is composed of O antigen, core, and lipid A. Molecular mass markers (in kilodaltons) are indicated on the left. Eight micrograms of A. pleuropneumoniae LPS was loaded in lanes 2 to 4.