FIG 3.
ELISpot assay data showing the number of IFN-γ-secreting cells detected in PBMCs isolated from pigs in the indicated groups on days 14 (2 weeks after priming), 21 (1 week after the boost), and 28 (2 weeks after the boost). Pigs in groups 1 and 2 (6 pigs each) were vaccinated with the 5 candidate proteins on days 0 and 14 of the experiment. Both groups were given the 5 proteins intranasally with polyethyleneimine as the adjuvant; in addition, group 1 pigs were given the 5 proteins intramuscularly with AddaVax and Carbopol as the adjuvant, while group 2 pigs were given the 5 proteins intramuscularly with Emulsigen-D as the adjuvant. Pigs in groups 3 to 5 were controls given the adjuvants alone (groups 3 and 4, 3 pigs each) or PBS (group 5, 4 pigs). PBMCs collected on days 14, 21, and 28 were seeded at 2.5 × 105 cells per well in duplicate and stimulated with a pool of the 5 candidate proteins. Control wells were stimulated with medium alone or pokeweed mitogen (data not shown). The means and standard errors of the means for each of the treatment groups are denoted. *, statistically significant differences between groups (P < 0.05).