FIG 5.

Survival rates of pigs vaccinated with 5 subunit proteins with different adjuvant formulations (groups 1 and 2) compared to those of pigs given adjuvant alone (groups 3 and 4) or PBS (group 5). Pigs in groups 1 and 2 (6 pigs each) were vaccinated with the 5 candidate proteins on days 0 and 14 of the experiment. Both groups were given the 5 proteins intranasally with polyethyleneimine as the adjuvant; in addition, group 1 pigs were given the 5 proteins intramuscularly with AddaVax and Carbopol as the adjuvant, while group 2 pigs were given the 5 proteins intramuscularly with Emulsigen-D as the adjuvant. Pigs in groups 3 to 5 were controls given the adjuvants alone (groups 3 and 4, 3 pigs each) or PBS (group 5, 4 pigs).