TABLE 6.
Primers used for protein cloning in this study
| Primer | Primer function | Sequencea (5′-3′) |
|---|---|---|
| 0185-4F | Cloning primers for SSU0185 | GACGACGACAAGATGTTCCGTTTAGCAAAAGAAGAAC |
| 0185-1167R | GAGGAGAAGCCCGGTTATTTTTCTAAAGGATGGATGA | |
| 1915-4F | Cloning primers for SSU1915 | GACGACGACAAGATGAAACACAATCTCCTTAAGAGCG |
| 1915-1257R | GAGGAGAAGCCCGGTTAGTTGCTGTGTTTTTGAGCAA | |
| 1215-82F | Cloning primers for SSU1215 | GACGACGACAAGATGGGCTTTATTATTGGGAAAGG |
| 1215-1831R | GAGGAGAAGCCCGGTTATTCTTTACTGGATTTTTTTC | |
| 1355-91F | Cloning primers for SSU1355 | GACGACGACAAGATGTTAGCTGTCCAAATTATGGGAG |
| 1355-2022R | GAGGAGAAGCCCGGTTACTCCCCTTCCTTACGTCTCA | |
| 1773-121F | Cloning primers for SSU1773 | GACGACGACAAGATGGATACTAGTGGAGAAGGATTGG |
| 1773-5076R | GAGGAGAAGCCCGGTTATTCTTTTCGCTTCAAATTTC |
a
Underlined nucleotides correspond to the sequence extensions required for LIC compatibility with the pET-30 Ek/LIC cloning vector.