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. 2018 Feb 20;86(3):e00662-17. doi: 10.1128/IAI.00662-17

FIG 3.

FIG 3

Identification of a genetic mutant (ima1Δ strain) with increased exo-GXM release but no observable changes to capsule thickness. (A) Representative india ink images of cells grown in 10% Sabouraud's dextrose medium, pH 7.3, for 24 h. Capsule thicknesses were similar across KN99 wild-type (WT) cells and cells from each independent ima1 mutant (also cnag_00658; see main text for details) deletion strain (ima1Δ #1 and ima1Δ #2). (B) Quantification of cell body diameter and capsule thickness from three independent experiments (n = 30 cells per strain; bars represent means with standard deviations). (C) Conditioned medium from cultures grown for 24 h under strong capsule-inducing conditions (10% Sabouraud's medium, pH 7.3). Blots were probed with anti-GXM antibody F12D2. (D) Quantification of blot signal intensities shows increased exo-GXM release by ima1Δ mutants 1/2 (combined data from three independent experiments). P values were calculated using a Mann-Whitney test; bars represent means with standard deviations. (E) Conditioned medium from cultures grown for 24 h under non-capsule-inducing conditions (YNB medium plus 2% glucose). Blots were probed with anti-GXM antibody F12D2. (F) Quantification of blot signal intensities shows similar levels of exo-GXM release by ima1Δ mutants 1/2 and wild-type cells (combined data from three independent experiments). P values were calculated using a Mann-Whitney test; bars represent means with standard deviations.