FIG 8.
Mice infected with liv7Δ cells display increased innate immune cell infiltrate in the brain. Mouse brains were harvested late (20 dpi) in infection for flow cytometry analysis of infiltrating immune cells. (A) CD4+ T cells are scarce in both wild-type- and liv7Δ mutant 1/2-infected brains. (B) CD8+ T cells show a significant increase over wild-type in liv7Δ mutant 2-infected brains, but this was not replicated in liv7Δ mutant 1-infected brains. Macrophages (CD45hi F4/80+) (C) and neutrophils (CD45+ Ly6G+ Ly6C+) (D) are significantly increased in the brains of liv7Δ mutant 1/2-infected mice compared to levels in wild-type- and mock-infected brains. P values were calculated using a Mann-Whitney test; bars represent the medians.