FIG 2.

(A) Schematic representation of S. sanguinis pilT genomic regions and the double-crossover event for generation of PilT-luciferase translational reporter strain. (B) Regulation of translational luciferase fusion of pilT by csRNA1-1 and csRNA1-2. PilT-luciferase reporter strains were grown in BHI broth to early exponential phase, and luciferase activity was measured. Data are normalized to the OD₆₀₀ of corresponding cultures and converted to percent luciferase activity relative to that of the WT-luc strain. Data are expressed as means ± SD from triplicate experiments. *, P < 0.01.