FIG 3.

Growth phase-dependent expression of PilT. PilT-luciferase reporter strains were grown over a 6-h period, and samples were collected for luciferase assay at an OD₆₀₀ of 0.1 to 0.2 (early log phase), 0.4 to 0.5 (mid-log phase), and 0.7 to 0.8 (late-log phase). (A) Growth curves of the strains over the measurement period. (B) PilT reporter activities of the strains. Luciferase activity was measured and divided by the OD₆₀₀ of corresponding cultures. Data are expressed as means ± SD of arbitrary units (AU) from three independent cultures. *, P < 0.01.