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Effect of csRNA1-1 and csRNA1-2 deletion on biofilm formation. S. sanguinis strains were grown in BHI broth containing 1% sucrose on saliva-coated 24-well polystyrene plates. Adherent and nonadherent cells were separately collected and quantified by measuring OD620. Percentages of adherent cells were calculated by the following equation: 100 × OD620 of adherent cells/OD620 of total (adherent and nonadherent) cells. *, P < 0.01.
