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. 2018 Feb 20;86(3):e00834-17. doi: 10.1128/IAI.00834-17

FIG 3.

FIG 3

IL-17 expression in response to recombinant IL-19 and an anti-IL-19 antibody. (a) Addition of recombinant IL-19 to PBMCs caused a small, insignificant decrease in IL-17 mRNA expression. Addition of recombinant IL-19 at 10 or 100 pg/ml to PBMC cultures stimulated with an exogenous strain decreased IL-17 mRNA expression compared to that in exogenous-strain-stimulated cultures with no addition (P < 0.0001). (b) Addition of IL-19 antibodies to PBMCs caused an insignificant increase in IL-17 mRNA expression, and addition of IL-19 antibodies at 0.8 μg/ml to PBMC cultures stimulated with an endogenous strain resulted in an increased IL-17 mRNA response compared to that of the endogenous-strain-stimulated culture with no addition (P < 0.0001). ANOVA with Tukey's post hoc analysis was done on all repeats to determine differences in IL-17 mRNA expression. (c) mRNA expression of IL-17 and IL-19 over 48 h in PBMCs isolated from one carrier in response to an endogenous strain.